Fluoresence Imaging

Fluoresence Imaging

EasyRatioPro allows researchers to reclaim control of their imaging research by reducing the learning curve associated with complex general purpose imaging platforms. The Easy Ratio Pro – Fluoresence Imaging introducing a bold advance in the field of microscope imaging by offering the most comprehensive high-speed multi-wavelength imaging system of its kind. It offers imaging researchers superb quality and efficiency through one intuitive, integrated production environment. In addition, it revolutionizes imaging by offering a unique hands-on user interface for unparalleled control. EasyRatioPro software represents the ultimate expandable platform for professional live cell imaging applications and offers by setting the standard for reliability, efficiency, and integration with a multi-wavelength illuminator.

Applications
Calcium Measurement
Intracellular free Ca2+, Mitochondrial Ca2+, Endoplasmic reticulum (ER) Ca2+pool, Lysosomal Ca2+, Extracellular near membrane Ca2+

Other Ions
Intracellular Mg2+, Intracellular Mn2+, Intracellular Zn2+, Intracellular Sr2+, Intracellular Na+, Intracellular Cl-, Cytosolic labile iron pool (LIP): Iron homeostasis

Membrane Potential
Plasma membrane Potential , Mitochondrial Membrane Potential

pH
Intracellular pH, Vacuolar pH, Lysosomal pH

GFP & Variants
Identifying cells coexpressing GFP for analysis, Use mitoGFP to visualize mitochondria and measure mitochondrial calcium [Ca2+]m, Use ratiometric GFP (redox-GFP) to measure cellular/mitochondria reduce/oxidize reaction, HEK-293 cells transfected with the CaSR-pEGFP vector, ECFP, EYFP

FRET
Donor Excitation Spectroscopy, Emission intensity, Donor emission intensity, Acceptor emission intensity, Ratio of donor/acceptor emission intensity, Measure molecular proximity, Binding of enzyme to its substrate, Measure Endoplasmic reticulum (ER) Ca2+

Other Measurements
Calcium imaging of Single HEK 293 cell, Taste Bud, Pea Pollen tubes, Respiratory burst & reactive oxygen species (ROS), NAD(P)H, Changes in CaM availability, Demonstration of selective plasma membrane permeabilization by digitonin in HEK293 cells, Detection by immunofluorescence of PfCRT expression in HEK293 cells, Receptor-mediated Glutamate release (by following NADH signal), Fura-dextra, Cellular GSH level, FL-aldosterone efflux, Latency of response to exogenous, ATP or ADP, Membrane traffic, Liposome, ER membrane, Cell contractile activity & cell Size, Cell Volume Measurement, Phagocytosis, Cell injury or death by PI fluorescent, Metal induced cytotoxity.